Saturday, March 3, 2012

5.13b Hosting recombinant DNA


- Recombinant DNA (human gene and Bacterial Plasmid) 
- transfer this structure to the host cell (virus) 
- inside the virus - nucleic acid (e.g. DNA or RNA) 
- around the virus - protein shell (capsid) 
- first thing - remove the nucleic acid from the virus to leave the capsid
- The plasmids are taken up by the virus. The virus acts as a vector for the recombinant DNA 
- This helps to transfer the DNA into the host cell 
- This virus is known as a phage (infects bacterial cells) 
- able to attach to the cell membrane of the bacteria and insert the recombinant DNA into the host cell
- at the end of the process: Bacterial cell which contains the recombinant DNA (including the human gene, insulin) 
- The bacteria has its own DNA plus the DNA from another organism - this combination is known as 'transgenic' 

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