5.20 - Cloned transgenic animals

- animals that are cloned are genetically identical 
- transgenic refers to an organism with DNA from 2 or more organisms 


- commercial production of antibodies 


-cow - obtain egg cell 
- human - take a cell - using restriction enzymes, going to take the 'gene antibody production' 


1. in the egg cell - first step is to knock out the cow antibody production gene 
2. Add human gene - transfer from human cell
3. Cows cell is then developed by mitosis to form a clone of cells - embryo - transferred to the surrogate mother 
4. These then produce genetically identical calves 


In this example, the gene for antibodies is expressed and the antibodies are then collected in the milk - this is the human antibody not cow - large commercial scale