Saturday, March 3, 2012

5.14 - Humulin


- Humulin - Human Insulin 

- Bacterial cell (e.g. E Coli) has been transformed by the addition of recombinant DNA 
- culture (large population) of the bacterial cell would be injected into the fermenter 
- the fermenter must provide the culture with nutrients, control of temperature, pH and the gases inside the fermenter
- by creating the optimal temperature for bacterial growth - you will see the population increase 
- the Bacteria switches on the gene for insulin and manufactures the protein Insulin from the provided Amino acids (in the nutrients)  
- then the product must be removed for purification - downstream processing (for human use) 
- the genetically engineered human insulin is called Humulin


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