Friday, March 9, 2012
5.19 - Mammal Cloning
sheep 1 = Original --genetically identical to clone--> dolly
- need to obtain genetic info from 1. - done by removing a diploid cell (has a full set of genetic information)
- at the same time - need to obtain a cell that has a tendency to divide
sheep 2. completely different animal - has been treated with hormones to produce eggs
- egg cell - tends to divide
- do not want the genetic information of the egg and so it is removed (enucleated)
- take the cell with the genetic information (want to copy) and the cell from the egg which wants to divide and FUSE them together
- when fusing them together we have the genetic information and a cell that wants to divide
- the combination of the two results in many cell divisions (like mitosis) to form a ball of cells (blastula - embryonic sheep - genetically identical to sheep 1)
-embryo (blastula) of sheep 1 is placed into another sheep 3 - which is the surrogate mother
- the embryo will grow into a fetus
- this fetus is the clone of sheep 1 and is what we call dolly - it is genetically identical to sheep 1
- this means they are a clone, even though their age is very different
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